| 產(chǎn)品編號(hào) | bs-10436R |
| 英文名稱(chēng) | Rabbit Anti-Rasgrp1 antibody |
| 中文名稱(chēng) | Ras蛋白特異鳥(niǎo)嘌呤核苷酸釋放因子1抗體 |
| 別 名 | Calcium and DAG-regulated guanine nucleotide exchange factor II; CALDAG-GEFI; CALDAG-GEFII; Guanine nucleotide exchange factor, calcium and DAG regulated, Rap1A; hRasGRP1; Ras activator RasGRP; RAS guanyl nucleotide releasing protein 1; RAS guanyl releasing protein 1 (calcium and DAG-regulated); RAS guanyl releasing protein 1; RASGRP; RASGRP1; GRP1_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 結(jié)合蛋白 G蛋白偶聯(lián)受體 G蛋白信號(hào) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | (predicted: Human,Mouse,Rat,Rabbit,Dog) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 90kDa |
| 細(xì)胞定位 | 細(xì)胞膜 細(xì)胞外基質(zhì) |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Rasgrp1: 701-797/797 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE). [provided by RefSeq, Jul 2008]. Function: RasGRP1 is a Ras-specific exchange factor, which is activated by T-cell receptor (TCR) and promotes TCR-dependent positive selection of thymocytes. RasGRP1 is highly expressed on most T lymphocytic leukemias and is a common site of proviral insertion in retrovirus-induced murine T-cell lymphomas. Subunit: Forms a signaling complex with DGKZ and HRAS. Interacts with F-actin. Interacts with SKAP1. Subcellular Location: Cell Membrane, Cytoplasmic, Endoplasmic reticulum and Golgi Apparatus. Found in membrane fraction. Relocalization to the cell membrane upon activation is F-actin-dependent. Translocates to the Golgi in response to phorbol ester or nerve growth factor. Tissue Specificity: Expressed in brain with higher expression in cerebellum, cerebral cortex and amygdala. Expressed in the hematopoietic system. Expressed in T-cells (at protein level). Post-translational modifications: Defects in RASGRP1 may contribute to susceptibility to systemic lupus erythematosus (SLE) [MIM:152700]. SLE is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. SLE is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Note=Aberrantly spliced isoforms and/or diminished levels of RASGRP1 are found in a cohort of SLE patients raising the possibility that dysregulation of this signaling protein contributes to the development of autoimmunity in a subset of SLE patients. Similarity: Belongs to the RASGRP family. Contains 2 EF-hand domains. Contains 1 N-terminal Ras-GEF domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 Ras-GEF domain. SWISS: O95267 Gene ID: 10125 Database links: Entrez Gene: 10125 Human Entrez Gene: 19419 Mouse Omim: 603962 Human SwissProt: O95267 Human SwissProt: Q9Z1S3 Mouse Unigene: 591127 Human Unigene: 42150 Mouse |
