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Rabbit Anti-NUDCD3  antibody (bs-13766R)  
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產(chǎn)品編號 bs-13766R
英文名稱 Rabbit Anti-NUDCD3  antibody
中文名稱 NUDCD3蛋白抗體
別    名 KIAA1068; NudC domain containing 3; NudC domain containing protein 3; NudC like protein; NUDCD 3; NudCL; OTTHUMP00000159457; NUDC3_HUMAN; NudC domain-containing protein 3..  
研究領(lǐng)域 細(xì)胞生物  發(fā)育生物學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Rat (predicted: Mouse,Pig)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 41 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NUDCD3: 161-260/361 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Nuclear migration is essential for growth, development and cellular function of multicellular organisms. NUDCD3 (NudC domain containing 3) is a 361 amino acid protein that contains one CS domain and is phosphorylated upon DNA damage by either ATR or ATM. Ubiquitously expressed, NUDCD3 has been observed to colocalize with Golgi marker proteins during interphase of mitosis in HeLa cells, and is found at low levels in brain, ovary and heart. The gene encoding NUDCD3 maps to human chromosome 7, which houses over 1,000 genes and comprises nearly 5% of the human genome. Chromosome 7 has been linked to Osteogenesis imperfecta, Pendred syndrome, Lissencephaly, Citrullinemia and Shwachman-Diamond syndrome.

Function:
NUDCD3 functions to maintain the stability of dynein intermediate chain. Depletion of NUDCD3 results in aggregation and degradation of dynein intermediate chain, mislocalization of the dynein complex from kinetochores, spindle microtubules, and spindle poles, and loss of gamma-tubulin from spindle poles. NUDCD3 levels increase after the G1/S transition.

Similarity:
Contains 1 CS domain.

SWISS:
Q8IVD9

Gene ID:
23386

Database links:

Entrez Gene: 23386 Human

Omim: 610296 Human

SwissProt: Q8IVD9 Human

Unigene: 488171 Human



產(chǎn)品圖片
Sample: Hl-60 Cell (Human) Lysate at 30 ug Primary: Anti- NUDCD3 (bs-13766R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kD
Sample: U937 Cell (Human) Lysate at 30 ug Primary: Anti- NUDCD3 (bs-13766R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUDCD3) Polyclonal Antibody, Unconjugated (bs-13766R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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