| 產(chǎn)品編號 | bs-7351R |
| 英文名稱 | CD200R Rabbit pAb |
| 中文名稱 | CD200受體抗體 |
| 別 名 | CD 200R; CD200 cell surface glycoprotein receptor; CD200 receptor 1; Cd200r1; Cell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; Cell surface glycoprotein OX2 receptor; cell surface glycoprotein receptor CD200; CRTR 2; C |
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Specific References (3) | bs-7351R has been referenced in 3 publications.
[IF=2.8] Clark, David A., et al. "Changes in expression of the CD200 tolerance‐signaling molecule and its receptor (CD200R) by villus trophoblasts during first trimester missed abortion and in chronic histiocytic intervillositis." American Journal of Reproductive Immunology (2017). IHC-P ; Human.
[IF=2.8] Clark, David A., et al. "The Receptor for the CD200 Tolerance‐Signaling Molecule Associated with Successful Pregnancy is Expressed by Early‐Stage Breast Cancer Cells in 80% of Patients and by Term Placental Trophoblasts." American Journal of Reproductive Immunology 74.5 (2015): 387-391. IHC-P ; Human.
[IF=2.8] Clark, David A., Jorge L. Arredondo, and Sukhbinder Dhesy-Thind. "The CD200 tolerance-signaling molecule and its receptor, CD200R1, are expressed in human placental villus trophoblast and in peri-implant decidua by 5 weeks’ gestation." Journal of Reproductive Immunology 112 (2015): 20-23. IHC-P ; Human.
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| 研究領域 | 細胞生物 免疫學 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD200R: 151-250/325 <Extracellular> |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a receptor for the OX-2 membrane glycoprotein. Both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. This receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. Mouse studies of a related gene suggest that this interaction may control myeloid function in a tissue-specific manner. Alternative splicing of this gene results in multiple transcript variants. Function: Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. Subcellular Location: Secreted and Cell membrane. Tissue Specificity: Expressed in granulocytes, monocytes, most T cells, neutrophils, basophils and a subset of NK, NKT and B cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells. Post-translational modifications: The mature form of isoform 2 and/or isoform 4 starts at sequence position 27 of the corresponding isoform. Similarity: Belongs to the CD200R family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: Q8TD46 Gene ID: 131450 Database links: Entrez Gene: 131450 Human Omim: 607546 Human SwissProt: Q8TD46 Human Unigene: 309158 Human |
| 產(chǎn)品圖片 |
Sample:hela(Human) Cell Lysate at 30 ug
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD200R (bs-7351R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 58 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CD200R) Polyclonal Antibody, Unconjugated (bs-7351R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Blank control:Hepg2 Cells(blue). Primary Antibody: Rabbit Anti-CD200R/AF647 Conjugated antibody (bs-7351R-AF647), Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
