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ATPIF1 Rabbit pAb (bs-12550R)  
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產(chǎn)品編號(hào) bs-12550R
英文名稱 ATPIF1 Rabbit pAb
中文名稱 ATPIF1蛋白抗體
別    名 ATIF1_HUMAN; ATP synthase inhibitor protein; ATPase inhibitor; ATPase inhibitor mitochondrial; ATPase inhibitor protein; ATPase inhibitory factor 1; ATPI; ATPIF 1; ATPIF1; ATPIP; IF(1); IF1; Inhibitor of F(1)F(o)-ATPase; IP; MGC1167; MGC8898; mitochondrial.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  信號(hào)轉(zhuǎn)導(dǎo)  新陳代謝  線粒體  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human (predicted: Mouse,Rat,Rabbit,Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 10 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ATPIF1/ATPase Inhibitory Factor 1: 26-106/106 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. F1 consists of three Alpha chains and three Beta chains, which are weakly homologous, as well as one Gamma chain, one Delta chain and one Gamma chain. F0 consists of three subunits: a, b and c. A mitochondrial F1-ATPase inhibitor protein, ATPIF1 (ATPase inhibitory factor 1), also known as IP, IF1, ATPI or ATPIP (ATPase inhibitor protein), binds to the C-terminal region of a Beta subunit of the F1-ATPase at low pH values and, via interference of the Beta and Gamma subunit interaction, ATPIF1 regulates the activity of the F1F0-ATPase. This reversible ATPIF1 binding to F1F0-ATPase also occurs on the surface of endothelial cells.

Function:
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.

Subunit:
Homodimer; represents the active form and is present at a pH value below 6.5. Homotetramer; represents the inactive form and is present at a pH value above 7.0 (By similarity).

Subcellular Location:
Mitochondrion.

Post-translational modifications:
Exhibits variability in chain length, mitochondria have distinct pools of protein cleaved after the 24th, 25th, and 26th amino acid.

Similarity:
Belongs to the ATPase inhibitor family.

SWISS:
Q9UII2

Gene ID:
93974

Database links:

Entrez Gene: 93974 Human

Entrez Gene: 11983 Mouse

Entrez Gene: 25392 Rat

Entrez Gene: 327699 Cow

SwissProt: P01096 Cow

SwissProt: Q9UII2 Human

SwissProt: O35143 Mouse

SwissProt: Q03344 Rat

Unigene: 59150 Cow

Unigene: 590908 Human

Unigene: 726089 Human

Unigene: 2171 Mouse

Unigene: 1658 Rat



產(chǎn)品圖片
Sample: Lane 1: Human HeLa cell lysates Lane 2: Human K562 cell lysates Lane 3: Human U937 cell lysates Lane 4: Human 293T cell lysates Primary: Anti-ATPIF1 (bs-12550R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 10 kDa Observed band size: 10 kDa
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