| 產品編號 | bs-12306R |
| 英文名稱 | ZFX Rabbit pAb |
| 中文名稱 | 鋅指蛋白X染色體蛋白抗體 |
| 別 名 | X-linked zinc finger protein; zfx; ZFX_HUMAN; Zinc finger protein, X-linked; Zinc Finger X Chromosomal Protein; Zinc finger X-chromosomal protein. |
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Specific References (2) | bs-12306R has been referenced in 2 publications.
[IF=2.886] Jiancong Lu. et al. Circ_0020123 Increases ZFX Expression to Facilitate Non-Small Cell Lung Cancer Progression by Sponging miR-142-3p. Cancer Manag Res. 2021; 13: 1687–1698 WB ; Human.
[IF=1.706] Yang et al. Zinc finger protein x-linked (ZFX) contributes to patient prognosis, cell proliferation and apoptosis in human laryngeal squamous cell carcinoma. (2016) Int.J.Clin.Exp.Pathol. 8:13886-99 IHC ; Human.
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| 研究領域 | 細胞生物 發(fā)育生物學 信號轉導 干細胞 鋅指蛋白 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat,Sheep,Cow,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 90 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZFX: 401-500/805 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Zfy1 (zinc finger protein 1, Y linked), also known Zfy-1 is a 782 amino acid nuclear protein belonging to the Krüppel C2H2-type zinc-finger protein family and the ZFX/ZFY subfamily. Containing thirteen C2H2-type zinc fingers, Zfy1 is expressed in the genital ridge and adult testis and may be a probable transcription activator. The gene encoding Zfy1 maps to mouse chromosome Y. Function: Probable transcriptional activator. Subcellular Location: Nucleus. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. ZFX/ZFY subfamily. Contains 13 C2H2-type zinc fingers. SWISS: P17010 Gene ID: 7543 Database links: Entrez Gene: 7543 Human Omim: 314980 Human SwissProt: P17010 Human Unigene: 336681 Human |
| 產品圖片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ZFX Polyclonal Antibody, Unconjugated(bs-12306R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
