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Rabbit Anti-Laminin alpha 4  antibody (bs-11055R)  
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產(chǎn)品編號 bs-11055R
英文名稱 Rabbit Anti-Laminin alpha 4  antibody
中文名稱 層粘蛋白α4抗體
別    名 LAMA3; LAMA4 1; LAMA4; LAMA4_HUMAN; Laminin alpha 4; Laminin alpha 4 chain; Laminin subunit alpha 4 precursor; Laminin subunit alpha-4; Laminin-14 subunit alpha; Laminin-8 subunit alpha; Laminin-9 subunit alpha; Lamininα4; Laminin α4; Laminin-α4; Laminin α 4.  
Specific References  (1)     |     bs-11055R has been referenced in 1 publications.
[IF=2.795] Wan Q et al. Overexpression of laminin α4 facilitates proliferation and migration of fibroblasts in knee arthrofibrosis by targeting canonical Shh/Gli1 signaling. Connect Tissue Res. 2020 Jun 10;1-11.  IHC-P ;  Rabbit.  
研究領域 腫瘤  神經(jīng)生物學  信號轉導  細胞粘附分子  細胞外基質  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human (predicted: Mouse,Rat,Rabbit,Sheep,Cow,Dog,Horse)
產(chǎn)品應用 ICC/IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 200 kDa
檢測分子量
細胞定位 細胞膜 細胞外基質 分泌型蛋白 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Laminin alpha 4: 901-1000/1823 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Laminins are essential and abundant structural non-collagenous glyco- proteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices (ECMs)) are polymers of laminins with stabilizing Type IV collagen networks, Nidogen, and several proteoglycans. Basement membranes are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells. Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each laminin is a heterotrimer of a, b, and g chain subunits that undergoes cell-secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by integrins, dystroglycan, and cognate laminin receptors. The human Laminin a-4 gene maps to chromosome 6q21 and is expressed in adult heart, lung, ovary, small and large intestines, liver, and placenta.

Function:
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Subunit:
Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-4 is a subunit of laminin-8 (laminin-411), laminin-9 (laminin-421) and laminin-14 (laminin-423).

Subcellular Location:
Secreted > extracellular space > extracellular matrix > basement membrane. Major component.

Tissue Specificity:
In adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn tissues is observed in certain mesenchymal cells in tissues such as smooth muscle and dermis.

Similarity:
Contains 4 laminin EGF-like domains.
Contains 5 laminin G-like domains.

SWISS:
Q16363

Gene ID:
3910

Database links:

Entrez Gene: 3910 Human

Entrez Gene: 16775 Mouse

SwissProt: Q16363 Human

SwissProt: P97927 Mouse




產(chǎn)品圖片
Tissue/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Laminin alpha 4) Polyclonal Antibody, Unconjugated (bs-11055R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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