| 產(chǎn)品編號(hào) | bs-11017R |
| 英文名稱 | Rabbit Anti-NPTX2 antibody |
| 中文名稱 | 神經(jīng)細(xì)胞穿透素2抗體 |
| 別 名 | apexin; NARP; Neuronal pentraxin 2; Neuronal pentraxin II; Neuronal pentraxin-2; NP 2; NP-II; NP2; NPII; NPTX2; NPTX2_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 神經(jīng)生物學(xué) 細(xì)胞粘附分子 細(xì)胞骨架 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse (predicted: Human,Rat,Sheep,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 46 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NPTX2: 165-265/431 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Long pentraxins are a family of highly conserved proteins that are expressed in the brain and central nervous system, and form multimeric complexes. Neuronal pentraxin 1 (NP1), NP2, and neuronal pentraxin receptor (NPR) are members of the long pentraxins that represent a neuronal uptake pathway that may function during synapse formation and remodeling. The NP1 gene is located on chromosome 17q25.3 and the protein product mediates the uptake of synaptic material, including the presynaptic snake venom toxin, taipoxin (3). NP2, whose function is unknown, is located on chromosome 7q22.1 and like NP1 contains several potential N-linked glycosylation sites. NPR is expressed on the cell membrane and can form heteropentamers with NP1 and NP2 that can be released from the cell membrane by proteolysis. Function: Likely to play role in the modification of cellular properties that underlie long-term plasticity. Binds to agar matrix in a calcium-dependent manner. Subunit: Homooligomer or heterooligomer (probably pentamer) with neuronal pentraxin receptor (NPTXR). Subcellular Location: Secreted. Tissue Specificity: Brain, pancreas, liver, heart and skeletal muscle. Highest levels are seen in the testis. Similarity: Contains 1 pentaxin domain. SWISS: P47972 Gene ID: 4885 Database links: Entrez Gene: 4885 Human Entrez Gene: 53324 Mouse Omim: 600750 Human SwissProt: P47972 Human SwissProt: O70340 Mouse Unigene: 3281 Human Unigene: 10099 Mouse Unigene: 162101 Rat |
| 產(chǎn)品圖片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-NPTX2 (bs-11017R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 46 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NPTX2) Polyclonal Antibody, Unconjugated (bs-11017R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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