| 產(chǎn)品編號 | bs-9856R |
| 英文名稱 | Rabbit Anti-HCN3 antibody |
| 中文名稱 | 鉀/鈉超極化激活環(huán)核苷酸門控通道蛋白3抗體 |
| 別 名 | Hcn3; HCN3_HUMAN; hyperpolarization activated cyclic nucleotide-gated potassium channel 3; KIAA1535; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3. |
| 研究領(lǐng)域 | 心血管 神經(jīng)生物學(xué) 信號轉(zhuǎn)導(dǎo) 通道蛋白 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat,Rabbit,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:50-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 86kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HCN3: 51-150/774 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Hyperpolarization-activated, cyclic nucleotide-binding channels (HCN) are voltage-gated cation channels that are activated by direct binding of intracellular cyclic nucleotides. The HCN family consists of four members (HCN1-4), each with a core transmembrane segment domain and a C-terminal 120 amino-acid cyclic nucleotide-binding domain motif. HCN channels are expressed in the brain, heart, thalamus and testis. The pacemaker properties of HCN channels contribute to spontaneous rhythmic activity in the brain and heart. HCN3 contains a segment characterized by a series of positively charged amino acids at every third position. This region designated S4 is likely to be the voltage sensor of the protein. In the brain, HCN3 and HCN4 exhibit subcortical distribution mainly concentrated in the hypothalamus and thalamus, respectively. Function: Putative hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions (By similarity). Subunit: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Subcellular Location: Membrane; Multi-pass membrane protein. Similarity: Belongs to the potassium channel HCN family. Contains 1 cyclic nucleotide-binding domain. SWISS: Q9P1Z3 Gene ID: 57657 Database links: Entrez Gene: 57657 Human Entrez Gene: 15168 Mouse Omim: 609973 Human SwissProt: Q9P1Z3 Human SwissProt: O88705 Mouse Unigene: 706960 Human Unigene: 389461 Mouse Unigene: 48801 Rat |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (APR3) Polyclonal Antibody, Unconjugated (bs-9856R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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