| 產(chǎn)品編號 | bs-9706R |
| 英文名稱 | JWA Rabbit pAb |
| 中文名稱 | JWA蛋白抗體 |
| 別 名 | Addicsin; ADP ribosylation like factor 6 interacting protein 5; Aip 5; Aip5; ARL 6 interacting protein 5; ARL6IP5 antibody Cytoskeleton related vitamin A responsive protein; Dermal papilla derived protein 11; DERP 11; DERP11; Glutamate transporter EEAC 1 |
| 研究領域 | 細胞生物 免疫學 細胞周期蛋白 轉錄調節(jié)因子 細胞分化 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 產(chǎn)品應用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human JWA: 101-188/188 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
JWA is a four-transmembrane environmental responsive protein which binds to the CC chemokine recepor 5 (CCR5), a major co-receptor for human immunodeficiency virus (HIV). JWA is involved in environmental stress-responsive pathways in K562 cells, an erythroleukemia cell line derived from patients with chronic myeloid leukemia. Environmental stressors to K562 cells such as heat shock, a higher temperature than the ideal body temperature of the organism from which the cell line was derived, and oxidative stress, the production of oxygen-centered free radicals, regulate and increase the expres-sion of JWA. This response to environmental stressors suggests similiarity of JWA to heat shock protein 70 (HSP70), which is upregulated by heat stress and toxic chemicals. Function: Regulates intracellular concentrations of taurine andglutamate. Negatively modulates SLC1A1/EAAC1 glutamate transportactivity by decreasing its affinity for glutamate. May be involvedin membrane traffic (By similarity). Subunit: Forms multimers. Binds to prenylated RAB1A and RAB3A, tothe neuronal glutamate transporter SLC1A1/EAAC1, and to theADP-ribosylation like factor ARL6, a member of the Ras superfamily.Does not bind to VAMP2 (By similarity). Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cytoplasm. Associated with microtubules. Similarity: Belongs to the PRA1 family. SWISS: O75915 Gene ID: 10550 Database links: Entrez Gene: 10550 Human Omim: 605709 Human SwissProt: O75915 Human |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
