| 產(chǎn)品編號 | bs-8029R |
| 英文名稱 | CNKSR2/KSR2 Rabbit pAb |
| 中文名稱 | Ras激酶抑制因子連接增強蛋白2抗體 |
| 別 名 | CNKR 2; CNK2; CNKSR2; Connector enhancer of kinase suppressor of Ras 2; Connector enhancer of KSR2; KIAA0902; KSR2; RP11 450P7.2; CNKR2_HUMAN. |
| 研究領域 | 細胞生物 免疫學 信號轉導 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 114 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CNKSR2 corresponding to amino acids 863-508.: 481-580/1034 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
CNKR2 may function as an adapter protein or regulator of Ras signaling pathways. Function: May function as an adapter protein or regulator of Ras signaling pathways. Subunit: Interacts with RAF1, RAB2L and RAL GTPase proteins. Subcellular Location: Cytoplasm. Membrane; Peripheral membrane protein. Post-translational modifications: Phosphorylated on tyrosine. Similarity: Belongs to the CNKSR family. Contains 1 CRIC domain. Contains 1 DUF1170 domain. Contains 1 PDZ (DHR) domain. Contains 1 PH domain. Contains 1 SAM (sterile alpha motif) domain. SWISS: Q8WXI2 Gene ID: 22866 Database links: Entrez Gene: 22866 Human Entrez Gene: 245684 Mouse SwissProt: Q8WXI2 Human SwissProt: Q80YA9 Mouse Unigene: 632794 Human Unigene: 197074 Mouse |
| 產(chǎn)品圖片 |
Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-CNKSR2/KSR2 (bs-8029R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114 kD
Observed band size: 114 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNKSR2/KSR2) Polyclonal Antibody, Unconjugated (bs-8029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CNKSR2/KSR2) Polyclonal Antibody, Unconjugated (bs-8029R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
