| 產(chǎn)品編號(hào) | bs-7872R |
| 英文名稱 | PPHLN1 Rabbit pAb |
| 中文名稱 | 脈周蛋白1抗體（胃癌抗原蛋白Ga50） |
| 別 名 | Gastric cancer antigen Ga50; Periphilin 1; Periphilin-1; PPHLN_HUMAN; PPHLN1. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞周期蛋白 細(xì)胞分化 細(xì)胞類型標(biāo)志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 53 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PPHLN1: 301-400/458 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation. Function: Involved in epithelial differentiation and contributes to epidermal integrity and barrier formation. Subunit: Homodimer. Interacts with PPL. Subcellular Location: Nucleus. Cytoplasm. Tissue Specificity: Ubiquitous. Post-translational modifications: Substrate of transglutaminase (in vitro). SWISS: Q8NEY8 Gene ID: 51535 Database links: UniProtKB/Swiss-Prot: Q8NEY8.2 The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]. |
| 產(chǎn)品圖片 |
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- PPHLN1 (bs-7872R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Sample:
HL60 (Human) Cell Lysate at 30 ug
Primary: Anti- PPHLN1 (bs-7872R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPHLN1) Polyclonal Antibody, Unconjugated (bs-7872R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPHLN1) Polyclonal Antibody, Unconjugated (bs-7872R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
