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CDCA8 Rabbit pAb (bs-7834R)  
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產(chǎn)品編號 bs-7834R
英文名稱 CDCA8 Rabbit pAb
中文名稱 細(xì)胞分裂周期蛋白8抗體
別    名 BOR; BOREA_HUMAN; Borealin; CDCA8; Cell division cycle associated 8; Cell division cycle-associated protein 8; Dasra B; Dasra-B; DasraB; FLJ10468; FLJ12042; hDasra-B; MESRGP; OTTHUMP00000004514; Pluripotent embryonic stem cell-related gene 3 protein.  
研究領(lǐng)域 細(xì)胞生物  細(xì)胞周期蛋白  細(xì)胞分化  表觀遺傳學(xué)  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human (predicted: Mouse,Rat,Rabbit,Cow,Bee,Horse)
產(chǎn)品應(yīng)用 IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 31 kDa
檢測分子量
細(xì)胞定位 細(xì)胞核 細(xì)胞漿 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Borealin/CDCA8: 20-120/280 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.

Function:
Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.

Subunit:
May form homooligomers and homodimers.

Subcellular Location:
Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere.

Post-translational modifications:
Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230.
Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.

Similarity:
Belongs to the borealin family.

SWISS:
Q53HL2

Gene ID:
55143

Database links:

Entrez Gene: 55143 Human

Entrez Gene: 52276 Mouse

Entrez Gene: 500545 Rat

Omim: 609977 Human

SwissProt: Q53HL2 Human

SwissProt: Q8BHX3 Mouse

SwissProt: Q6AXW0 Rat

Unigene: 524571 Human

Unigene: 28038 Mouse

Unigene: 14970 Rat



產(chǎn)品圖片
Tissue/cell: human endometrium carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Borealin/CDCA8 Polyclonal Antibody, Unconjugated(bs-7834R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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