| 產品編號 | bs-7026R |
| 英文名稱 | Rabbit Anti-HSPE1 antibody |
| 中文名稱 | 熱休克蛋白10/HSPE1抗體 |
| 別 名 | CH10_HUMAN; 10 kDa heat shock protein, mitochondrial; Hsp10; 10 kDa chaperonin; Chaperonin 10 (CPN10); Early-pregnancy factor (EPF); Early pregnancy factor; heat shock protein family E (Hsp10) member 1; Heat shock 10kD protein 1; mitochondrial; EPF; CPN10; GROES; HSP10; HSP 10; HSP-10; Cpn 10; HSPE 1; |
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Specific References (1) | bs-7026R has been referenced in 1 publications.
[IF=8.44] Zai, Zhuoyan. et al. Estrogen antagonizes ASIC1a-induced chondrocyte mitochondrial stress in rheumatoid arthritis. J TRANSL MED. 2022 Dec;20(1):1-14 IF ; Rat.
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| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉導 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human (predicted: Mouse,Rat) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 10kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human HSP10: 51-102/102 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Cellular localization; Mitochondrion matrix. Function: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Subcellular Location: Mitochondrion matrix. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the GroES chaperonin family. SWISS: P61604 Gene ID: 3336 Database links: Entrez Gene: 3336 Human Entrez Gene: 15528 Mouse Omim: 600141 Human SwissProt: P61604 Human SwissProt: Q64433 Mouse Unigene: 1197 Human Unigene: 215667 Mouse Unigene: 106093 Rat |
| 產品圖片 |
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSP10/CPN10 Polyclonal Antibody, Unconjugated(bs-7026R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
