| 產品編號 | bs-6566R |
| 英文名稱 | PRSS3 Rabbit pAb |
| 中文名稱 | 腦胰蛋白酶3抗體 |
| 別 名 | Brain trypsinogen; Mesotrypsin; Mesotrypsinogen; MTG; Pancreatic trypsinogen III; Protease, serine, 3; Protease, serine, 4(trypsin 4, brain); PRSS3; PRSS4; Serine protease 3; Serine protease 4; T9; TRY3; TRY3_HUMAN; TRY4; Trypsin 3; Trypsin III; Trypsin I |
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Specific References (1) | bs-6566R has been referenced in 1 publications.
[IF=3.77] Ma, Ruiqiong, et al. "PRSS3 expression is associated with tumor progression and poor prognosis in epithelial ovarian cancer." Gynecologic oncology 137.3 (2015): 546-552. IHC-P ; Human.
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| 研究領域 | 腫瘤 細胞生物 腫瘤細胞生物標志物 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 33 kDa |
| 檢測分子量 | |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRSS3: 101-200/304 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Function;
Digestive protease specialized for the degradation of trypsin inhibitors. Tissue specificity; Pancreas and brain. Sequence similarities; Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. Cellular localization; Secreted. Function: Digestive protease specialized for the degradation of trypsin inhibitors. Subcellular Location: Secreted. Tissue Specificity: Pancreas and brain. Similarity: Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain. SWISS: P35030 Gene ID: 5646 Database links: Entrez Gene: 5646 Human Entrez Gene: 22073 Mouse Omim: 613578 Human SwissProt: P35030 Human Unigene: 654513 Human Unigene: 425318 Mouse Unigene: 9817 Rat |
| 產品圖片 |
Sample:
A549(Human) Cell Lysate at 40 ug
Primary: Anti-PRSS3 (bs-6566R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 33 kD
Observed band size: 33 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRSS3) Polyclonal Antibody, Unconjugated (bs-6566R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRSS3) Polyclonal Antibody, Unconjugated (bs-6566R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
