| 產(chǎn)品編號 | bs-6180R |
| 英文名稱 | Rab3A+Rab3B+Rab3C+Rab3D Rabbit pAb |
| 中文名稱 | ras癌基因家族Rab3A--Rab3D抗體 |
| 別 名 | RAB3A; RAB3; RAB3A member RAS oncogene family; RAB3B; RAB3B member RAS oncogene family; RAB3C; RAB3C member RAS oncogene family; RAB3D member RAS oncogene family; RAD3D; Ras related protein Rab 3A; Ras related protein Rab 3B; Ras related protein Rab 3C; R |
| 研究領(lǐng)域 | 神經(jīng)生物學 信號轉(zhuǎn)導 轉(zhuǎn)錄調(diào)節(jié)因子 G蛋白偶聯(lián)受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 24-27 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Rab3A+Rab3B+Rab3C+Rab3D: 101-150/220 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Rab proteins belong to the Ras small GTPase superfamily. The GTP-binding Rab3 subfamily has four known isoforms: 3A, 3B, 3C and 3D. These proteins are expressed in the highest concentration in the brain and endocrine tissues and are involved in regulated exocytosis of hormones and neurotransmitters. Specifically Rab3's are thought to play a role in presynaptic vesicle trafficking and priming. Function: Involved in exocytosis by regulating a late step in synaptic vesicle fusion. Could play a role in neurotransmitter release by regulating membrane flow in the nerve terminal. Subunit: Heterodimer with RIMS2. Part of a ternary complex involving PCLO and EPAC2. Interacts with RPH3A and RPH3AL. Interacts with the exocyst complex through SEC15. Binds SYTL4 and RIMS1. Interacts with RAB3IP. Interacts with SGSM1 and SGSM3. Subcellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Tissue Specificity: Specifically expressed in brain. Similarity: Belongs to the small GTPase superfamily. Rab family. SWISS: P20336 Gene ID: 5864 Database links: Entrez Gene: 115827 Human Entrez Gene: 5864 Human Entrez Gene: 5865 Human Entrez Gene: 9545 Human Entrez Gene: 19339 Mouse Entrez Gene: 19340 Mouse Entrez Gene: 67295 Mouse Entrez Gene: 69908 Mouse Entrez Gene: 399230 Xenopus laevis Entrez Gene: 495404 Xenopus laevis Omim: 179490 Human Omim: 179510 Human Omim: 604350 Human SwissProt: O95716 Human SwissProt: P20336 Human SwissProt: P20337 Human SwissProt: Q5VUL2 Human SwissProt: Q96E17 Human SwissProt: P35276 Mouse SwissProt: P62823 Mouse SwissProt: P63011 Mouse SwissProt: Q0PD62 Mouse SwissProt: Q0PD63 Mouse SwissProt: Q9CZT8 Mouse SwissProt: Q9JME6 Mouse SwissProt: Q3KQA5 Xenopus laevis Unigene: 123072 Human Unigene: 27744 Human Unigene: 482173 Human Unigene: 8036 Human |
| 產(chǎn)品圖片 |
Sample: Eye (Mouse) Lysate at 40 ug
Primary: Anti-Rab3A+Rab3B+Rab3C+Rab3D (bs-6180R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 24-27 kD
Observed band size: 24 kD
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Rab3A+Rab3B+Rab3C+Rab3D) Polyclonal Antibody, Unconjugated (bs-6180R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
