| 產(chǎn)品編號 | bs-5889R |
| 英文名稱 | Rabbit Anti-Hyaluronidase3 antibody |
| 中文名稱 | 透明質(zhì)酸酶3/玻璃酸酶3抗體 |
| 別 名 | Hyaluronidase 3; HYAL3; Hyaluronidase-3; Hyaluronoglucosaminidase 3; LUCA14; LUCA3; Minna14; HYAL3_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 信號轉(zhuǎn)導 細胞表面分子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Sheep) |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47kDa |
| 細胞定位 | 細胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Hyaluronidase3: 51-150/417 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
HYAL3 is a protein which is similar in structure to hyaluronidases. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. However, this protein has not yet been shown to have hyaluronidase activity. Subcellular Location: Secreted (By similarity). Lysosome (By similarity). Tissue Specificity: Bone marrow, testis and kidney. Isoform 4 is detected in all bladder tumor and prostate tumor cells. Similarity: Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain. SWISS: O43820 Gene ID: 8372 Database links: Entrez Gene: 8372 Human Entrez Gene: 109685 Mouse Omim: 604038 Human SwissProt: O43820 Human SwissProt: Q8VEI3 Mouse Unigene: 129910 Human Unigene: 729310 Human Unigene: 447916 Mouse Unigene: 72927 Rat |
| 產(chǎn)品圖片 |
Sample:
Mouse(Kidney) Lysate at 40 ug
Primary: Anti- Hyaluronidase3 (bs-5889R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Stomach tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Stomach tissue lysates
Primary: Anti-Hyaluronidase3 (bs-5889R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 53 kDa
Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-Hyaluronidase3 antibody (bs-5889R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
