| 產品編號 | bs-5884R |
| 英文名稱 | Endomucin Rabbit pAb |
| 中文名稱 | 內皮粘蛋白EMCN抗體 |
| 別 名 | Endomucin 2; Mucin 14; EMCN; EMCN2; Endomucin; Endomucin-2; Endothelial sialomucin; Gastric cancer antigen Ga34; MUC-14; MUCEN_HUMAN; Mucin-14. |
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Specific References (5) | bs-5884R has been referenced in 5 publications.
[IF=16.806] Jie Mi. et al. Implantable Electrical Stimulation at Dorsal Root Ganglions Accelerates Osteoporotic Fracture Healing via Calcitonin Gene-Related Peptide. 2021 Oct 28 IHC ; Rat.
[IF=12.479] Weizhen Chen. et al. An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties. Biomaterials. 2021 Nov;:121260 IF ; Rat.
[IF=11.508] Qinyu Ma. et al. Osteoclast-derived apoptotic bodies couple bone resorption and formation in bone remodeling. Bone Res. 2021 Jan;9(1):1-12 IF,IHC ; Mouse.
[IF=11.322] Jinxiu Yu. et al. Promoting osseointegration of titanium by pH-responsive releasing of H2S and optimizing polarization time for macrophages. COMPOS PART B-ENG. 2023 Mar;253:110554 IHC,IF ; Rat.
[IF=8.09] Shinki Koyama. et al. Angio-osteogenic capacity of octacalcium phosphate co-precipitated with copper gluconate in rat calvaria critical-sized defect. Sci Technol Adv Mat. 2022;23(1):120-139 IHC ; Rat.
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| 研究領域 | 腫瘤 免疫學 信號轉導 糖蛋白 細胞骨架 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Cow,Dog) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 25 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Endomucin: 181-261/261 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. Function: Endothelial sialomucin, also called endomucin or mucin-like sialoglycoprotein, which interferes with the assembly of focal adhesion complexes and inhibits interaction between cells and the extracellular matrix. Subcellular Location: Secreted and Cell membrane. Membrane. Consistently localized to the plasma membrane and less abundantly to Golgi-like perinuclear stacks. Tissue Specificity: Expressed in heart, kidney and lung. Post-translational modifications: Highly O-glycosylated. Sialic acid-rich glycoprotein. SWISS: Q9ULC0 Gene ID: 51705 Database links: Entrez Gene: 51705 Human Omim: 608350 Human SwissProt: Q9ULC0 Human Unigene: 152913 Human |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Endomucin) Polyclonal Antibody, Unconjugated (bs-5884R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
