| 產(chǎn)品編號 | bs-5789R |
| 英文名稱 | GPR39 Rabbit pAb |
| 中文名稱 | G蛋白偶聯(lián)受體39抗體 |
| 別 名 | GPCR GPR39; G protein coupled receptor GPR39; G Protein-Coupled Receptor 39; G-protein coupled receptor 39; GPR 39; GPR39; GPR-39; MGC149541. |
| 研究領(lǐng)域 | 細胞生物 免疫學 信號轉(zhuǎn)導 細胞膜受體 G蛋白偶聯(lián)受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Rabbit,Cow,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR39.: 251-350/453 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
GPCR39 is an integral membrane protein, which belongs to family 1 of G-protein coupled receptors. GPCR39 expression has been documented in human brain and peripheral tissues. ESTs have been isolated from human tissue libraries, including normal eye, brain, heart, melanocyte, uterus, vessel, cancerous brain, germ cell, kidney, and ovary. Function: Zn(2+) acts as a agonist. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Its effect is mediated mainly through G(q)-alpha and G(12)/G(13) proteins. Involved in regulation of body weight, gastrointestinal mobility, hormone secretion and cell death. Subcellular Location: Multi-pass membrane protein Tissue Specificity: Expressed in many tissues, including the stomach, intestine and hypothalamus. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: O43194 Gene ID: 2863 Database links: Entrez Gene: 2863 Human Entrez Gene: 71111 Mouse Omim: 602886 Human SwissProt: O43194 Human SwissProt: Q5U431 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR39) Polyclonal Antibody, Unconjugated (bs-5789R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR39) Polyclonal Antibody, Unconjugated (bs-5789R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
