| 產(chǎn)品編號 | bs-5494R |
| 英文名稱 | Rabbit Anti-phospho-MAP4K1 (Ser171) antibody |
| 中文名稱 | 磷酸化造血祖細胞激酶抗體 |
| 別 名 | MAP4K1(phospho S171); Hematopoietic progenitor kinase 1; Hematopoietic progenitor kinase; HPK1; Human Hematopoietic Progenitor Kinase; M4K1_HUMAN; Map4k1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen Activated Protein Kinase Kinase Kinase Kinase 1; Mitogen-activated protein kinase kinase kinase kinase 1. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領域 | 免疫學 信號轉(zhuǎn)導 激酶和磷酸酶 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Rat (predicted: Mouse,Pig,Dog,Horse) |
| 產(chǎn)品應用 | IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 91kDa |
| 細胞定位 | 細胞漿 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human MAP4K1 around the phosphorylation site of Ser171: RL(p-S)FI |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Function: May play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Subunit: Interacts with MAP3K1. Tissue Specificity: Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine. Similarity: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CNH domain. Contains 1 protein kinase domain. SWISS: Q92918 Gene ID: 11184 Database links: Entrez Gene: 11184 Human Entrez Gene: 26411 Mouse Omim: 601983 Human SwissProt: Q92918 Human SwissProt: P70218 Mouse Unigene: 95424 Human Unigene: 148278 Mouse |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p-MAP4K1(Ser171)) Polyclonal Antibody, Unconjugated (bs5494R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Molt-4 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 20% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with phospho-MAP4K1 Antibody(bs-5494R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
