| 產品編號 | bs-3793R |
| 英文名稱 | CD75 Rabbit pAb |
| 中文名稱 | 唾液酸轉移酶1/α-2,6唾液酸轉移酶抗體 |
| 別 名 | 6 sialyltransferase; 6-sialyltransferase 1; 6-ST 1; alpha 2; alpha 2, 6 ST 1; alpha 2, 6 ST; B cell antigen CD75; B-cell antigen CD75; beta galactoside alpha 2, 6 sialyltransferase 1; beta-galactoside alpha-2; CMP N acetylneuraminate beta galactosamide al |
| 研究領域 | 細胞生物 免疫學 信號轉導 轉運蛋白 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 47 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD75: 301-406/406 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a type II membrane protein that catalyzes the transfer of sialic acid from CMP sialic acid to galactose-containing substrates. The encoded protein, which is normally found in the Golgi apparatus but which can be proteolytically processed to a soluble form, is involved in the generation of the cell surface carbohydrate determinants and differentiation antigens HB6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found for this gene. Function: Transfers sialic acid from the donor of substrate CMP-sialic acid to galactose containing acceptor substrates. Subcellular Location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Secreted. Note=Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid. Post-translational modifications: The soluble form derives from the membrane form by proteolytic processing. The HB-6, CDW75, and CD76 differentiation antigens are cell-surface carbohydrate determinants generated by this enzyme. Similarity: Belongs to the glycosyltransferase 29 family. SWISS: P15907 Gene ID: 6480 Database links: Entrez Gene: 6480 Human Entrez Gene: 20440 Mouse Omim: 109675 Human SwissProt: P15907 Human SwissProt: Q64685 Mouse Unigene: 207459 Human Unigene: 149029 Mouse Unigene: 54567 Rat |
| 產品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with CD75 polyclonal antibody, unconjugated (bs-3793R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti- CD75 (bs-3793R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
Sample:
MCF-7(Human) Cell Lysate at 30 ug
A549(Human) Cell Lysate at 30 ug
A431(Human) Cell Lysate at 30 ug
Siha(Human) Cell Lysate at 30 ug
Primary: Anti- CD75 (bs-3793R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kD
Observed band size: 48 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
