| 產(chǎn)品編號 | bs-5115R |
| 英文名稱 | Cytochrome b5 Rabbit pAb |
| 中文名稱 | 細胞色素b5抗體 |
| 別 名 | CYB 5; CYB 5A; CYB5; CYB5_HUMAN; CYB5A; Cytochrome b 5; Cytochrome b5(microsomal); Cytochrome b5; Cytochrome b5 type A(microsomal); Cytochrome b5 type A; MCB 5; MCB5; Microsomal cytochrome b5; Microsomal cytochrome b5 type A; Type 1 cyt b5; CYB5A. |
| 研究領域 | 腫瘤 細胞生物 免疫學 信號轉(zhuǎn)導 新陳代謝 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Sheep,Cow,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 15 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 細胞膜 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Cytochrome b5: 9-80/134 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] Function: Cytochrome b5 is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. Subcellular Location: Isoform 1: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side. Isoform 2: Cytoplasm. DISEASE: Methemoglobinemia CYB5A-related (METHB-CYB5A) [MIM:250790]: A form of methemoglobinemia, a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the cytochrome b5 family. Contains 1 cytochrome b5 heme-binding domain. SWISS: P00167 Gene ID: 1528 Database links: Entrez Gene: 1528 Human Entrez Gene: 109672 Mouse Omim: 250790 Human Omim: 613218 Human SwissProt: P00167 Human SwissProt: P56395 Mouse SwissProt: Q544Z9 Mouse Unigene: 465413 Human Unigene: 31018 Mouse Unigene: 1055 Rat |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Cytochrome b5 polyclonal antibody, unconjugated (bs-5115R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
