| 產(chǎn)品編號 | bs-3884R |
| 英文名稱 | Rabbit Anti-GPX4 antibody |
| 中文名稱 | 谷胱甘肽過氧化酶4抗體 |
| 別 名 | Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4_HUMAN; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase mitochondrial; snGPx; snPHGPx; Sperm nucleus glutathione peroxidase. |
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Specific References (18) | bs-3884R has been referenced in 18 publications.
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| 研究領(lǐng)域 | 腫瘤 細胞生物 免疫學 信號轉(zhuǎn)導(dǎo) 新陳代謝 線粒體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Pig,Sheep,Cow) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 22kDa |
| 細胞定位 | 細胞漿 線粒體 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glutathione Peroxidase 4: 101-197/197 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Glutathione peroxidases (Gpxs) are ubiquitously expressed proteins which catalyze the reduction of hydrogen peroxides and organic hydroperoxides by glutathione. There are several isoforms which differ in their primary structure and localization. The classical cytosolic/mitochondrial GPx1 (cGPx) is a selenium-dependent enzyme, first of the GPx family to be discovered. GPx2, also known as gastrointestinal GPx (GI-GPx), is an intracellular enzyme expressed only at the epithelium of the gastrointestinal tract. Extracellular plasma GPx (pGPx or GPx3) is mainly expressed by the kidney from where it is released into the blood circulation. Phospholipid hydroperoxide GPx4 (PH-GPx) expressed in most tissues, can reduce many hydroperoxides including hydroperoxides integrated in membranes, hydroperoxy lipids in low density lipoprotein or thymine. All mammalian GPx family members, except for the recently described Cys containing GPx3 and epididymis-specific secretory GPx (eGPx or GPx5) isoforms, possess selenocysteine at the active site. Function: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Subunit: Monomer. Has a tendency to form higher mass oligomers. Subcellular Location: Isoform Mitochondrial: Mitochondrion. Isoform Cytoplasmic: Cytoplasm. Tissue Specificity: Present primarily in testis. Similarity: Belongs to the glutathione peroxidase family. SWISS: P36969 Gene ID: 2879 Database links: Entrez Gene: 2879 Human Entrez Gene: 625249 Mouse Omim: 138322 Human SwissProt: P36969 Human SwissProt: O70325 Mouse Unigene: 433951 Human Unigene: 359573 Mouse Unigene: 3647 Rat 谷胱甘肽過氧化物酶（GSH-Px）是機體內(nèi)廣泛存在的一種重要的過氧化物分解酶。硒是GSH-Px酶系的組成成分，它能催化GSH變?yōu)镚SSG，使有毒的過氧化物還原成無毒的羥基化合物，同時促進H2O2的分解，從而保護細胞膜的結(jié)構(gòu)及功能不受過氧化物的干擾及損害。GSH-Px的活性中心是硒半胱氨酸，其活力大小可以反映機體硒水平。GSH-Px酶系主要包括4種不同的GSH-Px，分別為胞漿GSH-Px、血漿GSH-Px、磷脂氫過氧化物GSH-Px及胃腸道專屬性GSH-Px。胞漿GSH-Px由4個相同的分子量大小為22kDa的亞基構(gòu)成四聚體，每個亞基含有1個分子硒半胱氨酸，廣泛存在于機體內(nèi)各個組織，以肝臟紅細胞為最多。它的生理功能主要是催化GSH參與過氧化反應(yīng)，清除在細胞呼吸代謝過程中產(chǎn)生的過氧化物和羥自由基，從而減輕細胞膜多不飽和脂肪酸的過氧化作用。 |
| 產(chǎn)品圖片 |
Sample: Kidney (mouse) Lysate at 40 ug
Primary: Anti-GPX4 (bs-3884R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22kD
Observed band size: 20 kD
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-GPX4 (bs-3884R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 19 kD
Sample:
Lane 1: Testis (Mouse) Tissue Lysate at 40 ug
Lane 2: Liver (Mouse) Tissue Lysate at 40 ug
Lane 3: Kidney (Mouse) Tissue Lysate at 40 ug
Lane 4: Heart (Mouse) Tissue Lysate at 40 ug
Lane 5: Testis (Rat) Tissue Lysate at 40 ug
Lane 6: Liver (Rat) Tissue Lysate at 40 ug
Lane 7: Kidney (Rat) Tissue Lysate at 40 ug
Lane 8: Heart (Rat) Tissue Lysate at 40 ug
Lane 9: U937 (Human) Cell Lysate at 30 ug
Lane 10: HepG2 (Human) Cell Lysate at 30 ug
Lane 11: MCF-7 (Human) Cell Lysate at 30 ug
Lane 12: Huvec (Human) Cell Lysate at 30 ug
Lane 13: 293T (Human) Cell Lysate at 30 ug
Primary: Anti-GPX4 (bs-3884R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 20-22 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
