| 產(chǎn)品編號(hào) | bs-2578R |
| 英文名稱 | Granzyme A Rabbit pAb |
| 中文名稱 | 顆粒酶A抗體 |
| 別 名 | CTL tryptase; CTLA3; Cytolytic t cell and natural killer cell specific trypsin like serine protease; Cytotoxic T lymphocyte associated serine esterase 3; Cytotoxic T lymphocyte proteinase 1; Cytotoxic T-lymphocyte proteinase 1; Fragmentin 1; Fragmentin-1; |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞凋亡 t-淋巴細(xì)胞 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 26 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GZMA: 101-200/262 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008] Function: This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Involved in apoptosis. Subunit: Homodimer; disulfide-linked. Interacts with APEX1. Subcellular Location: Isoform alpha: Secreted. Cytoplasmic granule. Similarity: Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain. SWISS: P12544 Gene ID: 3001 Database links: Entrez Gene: 3001 Human Omim: 140050 Human SwissProt: P12544 Human Unigene: 90708 Human GzmA是存在于細(xì)胞毒性T淋巴細(xì)胞(CTL)和自然殺傷細(xì)胞(NK細(xì)胞)的細(xì)胞毒顆粒中含量最多的一種絲氨酸蛋白酶。 |
| 產(chǎn)品圖片 |
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti- Granzyme A Polyclonal Antibody, Unconjugated(bs-2578R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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