| 產(chǎn)品編號 | bs-2490R |
| 英文名稱 | CD23 Rabbit pAb |
| 中文名稱 | CD23/FcεRII抗體 |
| 別 名 | Fc EpsilonR II; FcεRII; Blast 2; Blast2; C type lectin domain family 4 member J; C-type lectin domain family 4, member J; CD 23; CD 23A; CD23 antigen; CD23A; CLEC 4J; CLEC4J; Fc epsilon RII; Fc fragment of IgE low affinity II receptor for; Fc fragment of |
| 研究領(lǐng)域 | 細胞生物 免疫學 干細胞 細胞類型標志物 淋巴細胞 t-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse (predicted: Rat,Rabbit,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 36 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD23: 221-321/321 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011] Function: Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen). Subcellular Location: Cell membrane; Single-pass type II membrane protein. Secreted. Note=Also exists as a soluble excreted form. Post-translational modifications: N- and O-glycosylated. Similarity: Contains 1 C-type lectin domain. SWISS: P06734 Gene ID: 2208 Database links: Entrez Gene: 2208 Human Entrez Gene: 14128 Mouse Omim: 151445 Human SwissProt: P06734 Human SwissProt: P20693 Mouse Unigene: 465778 Human Unigene: 1233 Mouse CD23是一種II型跨膜糖蛋白，是存在于B細胞表面的IgE受體。在慢性B淋巴細胞白血?。–LL）和小B細胞淋巴瘤（SLL）為陽性，套細胞淋巴瘤和小核裂淋巴細胞淋巴瘤陰性。也表達于外周血B細胞的某些亞型、B淋巴細胞和EB病毒轉(zhuǎn)染的B淋巴瘤細胞系。CD23是慢性B淋巴細胞白血病，小B細胞淋巴瘤診斷和鑒別診斷有用的標記物。 |
| 產(chǎn)品圖片 |
Sample: 293T Cell (Human) Lysate at 30 ug Primary: Anti- CD23 (bs-2490R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 50/40 kD
Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti- CD23 (bs-2490R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 50/40 kD
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
