| 產品編號 | bs-1967R |
| 英文名稱 | Rabbit Anti-KLK9 antibody |
| 中文名稱 | 激肽釋放酶9抗體（舒血管素9） |
| 別 名 | Kallikrein 8; Kallikrein 9; Kallikrein L3; Kallikrein Like 3; Kallikrein like protein 3; kallikrein related peptidase 9; Kallikrein-9; Kallikrein-like protein 3; Kallikrein8; Kallikrein9; KLK 8; KLK 9; KLK L3; KLK-L3; KLK8; KLK9; KLK9_HUMAN; KLKL 3; KLKL3. |
| 研究領域 | 腫瘤 細胞生物 信號轉導 激酶和磷酸酶 細胞骨架 細胞外基質 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse (predicted: Human,Rat,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 26kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLK9: 51-150/250 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Kallikrein 9, also known as Kallikrein-Like 3 (KLK-L3), is a chymotrypsin-like serine proteinase. Kallikrein 9 was discovered as the locus for kallikreins on chromosome 19 was more fully mapped and found by similarity to the other tissue kallikreins. Kallikrein 9 has been found in the ovary, thymus, testis, prostate, skin, breast and neuronal tissues and is made by many cell lines in culture. Kallikrein 9 levels in breast cancer and uterine cancer patients have been reported to drop as the disease progresses, thus hK9 might be considered a favorable prognostic marker. Different splice variants of hK9 have been reported, although it is not yet known if they produce functional proteins. The full length Kallikrein 9 encodes for a 250 amino acid protein, with a predicted mass of 27.5 kDa and a pI of 7.53. The 234 amino acid form predicts a protein of 26 kDa with a pI of 9.76 and this quite basic pI might give the shorter form a very different function or localization. The shorter sequence also diverges before the catalytic serine residue, making it unlikely to be proteolytically active. Pre-pro-kallikrein 9 has the 17 amino acid signal sequence is removed before secretion, and the Pro-kallikrein 9 is activated to Kallikrein 9 by removal of the 5 amino acid propeptide domain. Subcellular Location: Secreted (Probable). Tissue Specificity: Skin, thymus, trachea, cerebellum and spinal cord. Similarity: Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain. SWISS: Q9UKQ9 Gene ID: 284366 Database links: Entrez Gene: 284366 Human Omim: 605504 Human SwissProt: Q9UKQ9 Human Unigene: 448942 Human KLK9目前認為是卵巢癌 宮頸癌 子宮內膜癌等其他腫瘤,很有意義的標志物。激肽釋放酶KLK是激肽系統(tǒng)的主要限速酶，它是一組存在于多數(shù)組織和體液中的絲氨酸蛋白酶，是一種肽鏈內切酶。KLK又稱血管舒緩素，包括15個家族成員。在不同的組織中廣泛表達,具有蛋白水解酶的活性。它特異性的在碳末端切割底物肽，可裂解激肽原釋放具有活性的激肽，由激肽發(fā)揮對心血管系統(tǒng)及腎臟功能的調節(jié)作用。 組織KLK是一個大的基因家族，主要分布在肺、腎、血管、腦、腎上腺組織，為一種中等大小的糖蛋白。 |
| 產品圖片 |
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (KLK9) Polyclonal Antibody, Unconjugated (bs-1967R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
