| 產(chǎn)品編號 | bs-1479R |
| 英文名稱 | CD80 Rabbit pAb |
| 中文名稱 | 刺激分子B7-1蛋白抗體 |
| 別 名 | Activation B7-1 antigen; B lymphocyte activation antigen B7; B7; B7-1; B7-1 antigen; BB1; CD28 antigen ligand 1; CD28LG; CD28LG1; CD80 antigen(CD28 antigen ligand 1, B7-1 antigen); CD80 antigen; CD80 molecule; CD80_HUMAN; Costimulatory factor CD80; costim |
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Specific References (6) | bs-1479R has been referenced in 6 publications.
[IF=9.207] Li Yin. et al. G6PD activation in TNBC cells induces macrophage recruitment and M2 polarization to promote tumor progression. CELL MOL LIFE SCI. 2023 Jun;80(6):1-20 WB ; Human.
[IF=8.897] Liu, Houli. et al. Immunomodulatory hybrid bio-nanovesicle for self-promoted photodynamic therapy. Nano Res. 2022 Feb;:1-10 WB ; Mouse.
[IF=3.457] Zheng X et al. Dendritic cells and Th17/Treg ratio play critical roles in pathogenic process of chronic obstructive pulmonary disease. (2018) Biomedicine & Pharmacotherapy.108,1141–1151. IHC ; Human.
[IF=3.427] Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900 FCM ; Human.
[IF=3.361] Ma M et al. Low-dose naltrexone inhibits colorectal cancer progression and promotes apoptosis by increasing M1-type macrophages and activating the Bax/Bcl-2/caspase-3 … Int Immunopharmacol. 2020 Mar 11;83:106388. IHC ; mouse.
[IF=1.48] Zhang, Suxin, et al. "Variation and significance of secretory immunoglobulin A, interleukin 6 and dendritic cells in oral cancer." Oncology Letters. IHC-P ; Human.
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| 研究領域 | 腫瘤 免疫學 干細胞 淋巴細胞 b-淋巴細胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=2μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 32 kDa |
| 檢測分子量 | 60-65 |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD80: 111-265/288 <Extracellular> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011] Function: Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. Subcellular Location: Membrane. Tissue Specificity: Expressed on activated B-cells, macrophages and dendritic cells. Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P33681 Gene ID: 941 Database links: Entrez Gene: 941 Human Entrez Gene: 12519 Mouse Omim: 112203 Human SwissProt: P33681 Human SwissProt: Q00609 Mouse Unigene: 838 Human Unigene: 89474 Mouse |
| 產(chǎn)品圖片 |
Sample:
HL-60(Human) Cell Lysate at 40 ug
Primary: Anti-CD80 (bs-1479R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 32 kD
Observed band size: 57 kD
Sample:
Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 ug
Lane 2: SP2/0 (Mouse) Cell Lysate at 30 ug
Lane 3: Thymus (Rat) Lysate at 40 ug
Lane 4: Raji (Human) Cell Lysate at 30 ug
Lane 5: K562 (Human) Cell Lysate at 30 ug
Primary: Anti-CD80 (bs-1479R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60-65 kD
Observed band size: 60 kD
Blank control: U937(blue). Primary Antibody: Rabbit Anti-CD80 antibody(bs-1479R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions. Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (bs-1479R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Blank control:Raji.
Primary Antibody (green line): Rabbit Anti-CD80 antibody (bs-1479R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
