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Rabbit Anti-Prohibitin  antibody (bs-1449R)  
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產(chǎn)品編號 bs-1449R
英文名稱 Rabbit Anti-Prohibitin  antibody
中文名稱 阻抑素/腫瘤抑制因子抗體
別    名 Mitochondrial Marker; PHB; PHB1; Prohibitin-Mitochondrial Marker; PHB_HUMAN.  
Specific References  (2)     |     bs-1449R has been referenced in 2 publications.
[IF=5.23] Wang, Jinzi, et al. "Comparative Secretome Analysis Reveals Perturbation of Host Secretion Pathways by a Hypovirus." Scientific Reports 6 (2016): 34308.  WB ;  Other Species.  
[IF=2.08] Xing, Wen Min, et al. "Proteomic identification of mitochondrial targets involved in andrographolide sodium bisulfite-induced nephrotoxicity in a rat model."Environmental Toxicology and Pharmacology (2015).  WB ;  Rat.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  染色質(zhì)和核信號  細(xì)胞凋亡  細(xì)胞類型標(biāo)志物  線粒體  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat (predicted: Dog,Horse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/test,ICC/IF=1:100
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 30 kDa
檢測分子量
細(xì)胞定位 細(xì)胞漿 線粒體
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human Prohibitin: 171-272/272 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Function:
Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging.

Subunit:
Interacts with PHB2. Interacts with STOML2.

Subcellular Location:
Mitochondrion inner membrane.

Tissue Specificity:
Widely expressed in different tissues.

Similarity:
Belongs to the prohibitin family.

SWISS:
P35232

Gene ID:
5245

Database links:

Entrez Gene: 5245 Human

Entrez Gene: 18673 Mouse

Entrez Gene: 25344 Rat

Omim: 176705 Human

SwissProt: P35232 Human

SwissProt: P67778 Mouse

SwissProt: P67779 Rat

Unigene: 514303 Human

Unigene: 263862 Mouse

Unigene: 29754 Rat



Prohibitin(phb)基因是一種有效的腫瘤抑制基因,廣泛分布于不同種屬的各種生物細(xì)胞中,它所編碼的產(chǎn)物Prohibitin(PHB)蛋白結(jié)構(gòu)保守,既存在于線粒體內(nèi)膜上,發(fā)揮分子伴侶作用,也存在于細(xì)胞核內(nèi),具有負(fù)性轉(zhuǎn)錄調(diào)控作用,因而對細(xì)胞代謝、生長、分化、衰老以及凋亡等諸多方面發(fā)揮 著重要的調(diào)控作用,可能與某些腫瘤和退行性疾病的發(fā)生有關(guān)。
產(chǎn)品圖片
Sample: Heart(Mouse) Lysate at 40 ug Primary: Anti-Prohibitin (bs-1449R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD
Sample: A431(Human) Cell Lysate at 40 ug Primary: Anti-Prohibitin (bs-1449R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 30 kD
Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated(bs-1449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Prohibitin Polyclonal Antibody, Unconjugated(bs-1449R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Prohibitin) polyclonal Antibody, Unconjugated (bs-1449R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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