| 產品編號 | bs-1294R |
| 英文名稱 | ODC1 Rabbit pAb |
| 中文名稱 | 鳥氨酸脫羧酶抗體 |
| 別 名 | Ornithine decarboxylase 1; Ornithine decarboxylase; Dodc1; Odc 1; ODC1; ODC2; Ornithine decarboxylase 2; DCOR_HUMAN; Ornithine decarboxylase 1; Ornithine decarboxylase; Ornithine decarboxylase structural 1; RNODC. |
 |
Specific References (5) | bs-1294R has been referenced in 5 publications.
[IF=4.492] Jing Wanget al. Regulatory role of l-proline in fetal pig growth and intestinal epithelial cell proliferation. Anim Nutr
. 2020 Dec;6(4):438-446. WB ; pig.
[IF=3.789] Wang, Hongliang. et al. A new 68Ga-labeled ornithine derivative for PET imaging of ornithine metabolism in tumors. AMINO ACIDS. 2023 Feb;:1-12 IHC ; Mouse.
[IF=2.83] Chen, Shaokui, et al. "Asparagine improves intestinal integrity, inhibits TLR4 and NOD signaling, and differently regulates p38 and ERK1/2 signaling in weanling piglets after LPS challenge." Innate Immunity (2016): 1753425916664124. WB ; Pig.
[IF=2.48] Rossi et al. Immunohistochemical expression of ornithine decarboxylase, diamine oxidase, putrescine, and spermine in normal canine enterocolic mucosa, in chronic colitis, and in colorectal cancer. (2015) Biomed.Res.In. 2015:172756 IHC ; dog.
[IF=1.58] Rossi, Giacomo, et al. "Immunohistochemical Expression of Ornithine Decarboxylase, Diamine Oxidase, Putrescine, and Spermine in Normal Canine Enterocolic Mucosa, in Chronic Colitis, and in Colorectal Cancer." BioMed Research International 2015 (2015). IHC-P ; Dog.
|
| 研究領域 | 腫瘤 信號轉導 新陳代謝 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Mouse,Rat (predicted: Human,Pig,Cow,Dog,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 51 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ODC1: 321-461/461 |
| 亞 型 | |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
Ornithine Decarboxylase (ODC) is the initial and rate limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa. Subunit: Homodimer. Post-translational modifications: S-Nitrosylation inhibits the enzyme. S-Nitrosylated in vitro on 4 cysteine residues. Similarity: Belongs to the Orn/Lys/Arg decarboxylase class-II family. SWISS: P11926 Gene ID: 4953 Database links: Entrez Gene: 4953 Human Entrez Gene: 18263 Mouse Omim: 165640 Human SwissProt: P11926 Human SwissProt: P00860 Mouse Unigene: 7133 Cow Unigene: 467701 Human Unigene: 34102 Mouse Unigene: 874 Rat 鳥氨酸脫羧酶為多胺生物合成途徑中的第一個限速酶 (催化合成多胺的第一步反應 ,即催化L -鳥氨酸脫羧生成腐胺 ) ,具有超常轉變率和對于刺激的迅速反應性。 多胺廣泛存在于生物體的各種細胞和組織中 ,是一種重要的代謝調控物質 ,它可直接與DNA相互作用 ,調節(jié)DNA構象 ,并參與RNA及蛋白質的合成 ,與細胞增殖、分化密切相關。經研究表明 ,哺乳動物細胞增殖體現出完全的多胺依賴性。ODC主要存在于胞漿中。 |
| 產品圖片 |
Sample: Kidney(Mouse) lysate at 30ug;
Primary: Anti-ODC(bs-1294R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:51 kD
Observed band size:51 kD
Sample:Liver(Mouse) lysate at 30ug;
Primary: Anti-ODC(bs-1294R) at 1:300 dilution;
Secondary: AP conjugated Goat Anti-Rabbit IgG(bs-0295G-AP) at 1: 5000 dilution;
Predicted band size : 51kD
Observed band size : 51kD
Sample: Kidney(Mouse) lysate at 30ug;
Primary: Anti-ODC(bs-1294R) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution;
Predicted band size:51 kD
Observed band size:51 kD
Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (bs-1294R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ODC) Polyclonal Antibody, Unconjugated (bs-1294R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
