| 產(chǎn)品編號 | bs-1094R |
| 英文名稱 | kappa Opioid receptor Rabbit pAb |
| 中文名稱 | kappa型阿片受體抗體 |
| 別 名 | Kappa type opioid receptor; KOR 1; KOR; Opiate receptor kappa 1; OPRK 1; OPRK; OPRK1; Oprk2; R21; OPRK_HUMAN. |
| 研究領域 | 細胞生物 免疫學 神經(jīng)生物學 細胞膜受體 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse,Rat |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 43 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human kappa Opioid receptor: 201-300/380 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Opioid is the term used to designate a group of compounds that are opium-like in their properties. These drugs have effects on perception of pain, consciousness, motor control, mood, and autonomic function, and can induce physical dependence. Pharmacological studies suggested that there are at least 3 major classes of opioid receptors, designated delta, kappa, and mu. They differ in their affinity for various opioid ligands and in their cellular distribution. Studies of the receptors in the mouse and rat show that they are structurally related and are members of the family of 7 transmembrane-spanning G protein-coupled receptors.
The kappa opiod receptor inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. It is the receptor for beta-endorphin. Function: Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions. Subunit: Interacts with SLC9A3R1. Interacts with GABARAPL1. Subcellular Location: Cell membrane; Multi-pass membrane protein. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P41145 Gene ID: 4986 Database links: Entrez Gene: 4986 Human Omim: 165196 Human SwissProt: P41145 Human Unigene: 106795 Human kappa型阿片受體在人腦和胎盤組織中有分布。κ- 受體參與鎮(zhèn)痛 ，且與神經(jīng)內(nèi)分泌及免疫調(diào)節(jié)有關。此外，κ受體也調(diào)控噴他佐辛樣脊髓鎮(zhèn)痛、鎮(zhèn)靜和瞳孔縮小。κ-阿片受體由380個氨基酸組成，同樣屬于G蛋白偶聯(lián)受體家族。 |
| 產(chǎn)品圖片 |
Sample: A549 Cell Lysate at 40 ug
Primary: rabbit Anti-kappa Opioid receptor (bs-1094R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size: 43 kD
Observed band size: 43 kD
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Rat Cerebellum tissue lysates
Primary: Anti-kappa Opioid receptor (bs-1094R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 45 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (kappa Opioid receptor) Polyclonal Antibody, Unconjugated (bs-1094R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-kappa Opioid receptor Polyclonal Antibody, Unconjugated(bs-1094R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
