| 產(chǎn)品編號(hào) | bs-0071R |
| 英文名稱(chēng) | Neuropeptide Y Rabbit pAb |
| 中文名稱(chēng) | 神經(jīng)肽Y抗體 |
| 別 名 | Neuropeptide tyrosine; Neuropeptide Y precursor; NPY; PYY 4; PYY4; Y Neuropeptide; Pro-neuropeptide Y; NPY_HUMAN. |
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Specific References (5) | bs-0071R has been referenced in 5 publications.
[IF=4.546] Quancheng Liu. et al. FumDSB Can Reduce the Toxic Effects of Fumonisin B1 by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs. Toxins. 2021 Dec;13(12):874 WB,IHC ; Pig.
[IF=3.23] Chen, Guiqian, et al. "The Stimulatory Effect of Cerebral Intraventricular Injection of cNPY on Precocial Feeding Behavior in Neonatal Chicks (Gallus domesticus)." PloS one 11.4 (2016): e0153342. other ; Chicken.
[IF=2.23] Dong et al. Anti-epileptic effects of neuropeptide Y gene transfection into the rat brain. (2013) Neural.Regen.Res. 8:1307-15 WB ; Rat.
[IF=2.02] Duan, Hubin, et al. "The role of neuropeptide Y and aquaporin 4 in the pathogenesis of intestinal dysfunction caused by traumatic brain injury." Journal of Surgical Research (2013). IHC-P ; Rat.
[IF=1.89] Chen, Shengru, et al. "Changes in nerve fibers and microvessel density in residual spleen after subtotal splenectomy due to portal hypertension." International Journal of Clinical and Experimental Pathology 9.2 (2016): 1557-1567. IHC-P ; Human.
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| 研究領(lǐng)域 | 心血管 信號(hào)轉(zhuǎn)導(dǎo) |
| 抗體來(lái)源 | Rabbit |
| 克隆類(lèi)型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Cow,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 4/8 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NPY: 29-64/97 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a neuropeptide that is widely expressed in the central nervous system and influences many physiological processes, including cortical excitability, stress response, food intake, circadian rhythms, and cardiovascular function. The neuropeptide functions through G protein-coupled receptors to inhibit adenylyl cyclase, activate mitogen-activated protein kinase (MAPK), regulate intracellular calcium levels, and activate potassium channels. A polymorphism in this gene resulting in a change of leucine 7 to proline in the signal peptide is associated with elevated cholesterol levels, higher alcohol consumption, and may be a risk factor for various metabolic and cardiovascular diseases. The protein also exhibits antimicrobial activity against bacteria and fungi. [provided by RefSeq, Oct 2014] Function: NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone. Subcellular Location: Secreted. Tissue Specificity: One of the most abundant peptides in the nervous system. Also found in some chromaffin cells of the adrenal medulla. Post-translational modifications: The neuropeptide Y form is cleaved at Pro-30 by the prolyl endopeptidase FAP (seprase) activity (in vitro). Similarity: Belongs to the NPY family. Gene ID: 4852 Database links: Entrez Gene: 396464 Chicken Entrez Gene: 4852 Human Entrez Gene: 109648 Mouse Omim: 162640 Human 神經(jīng)生物學(xué)相關(guān)蛋白 神經(jīng)肽Y是一種神經(jīng)傳導(dǎo)因子，參與機(jī)體的應(yīng)急反應(yīng)， 在應(yīng)激狀態(tài)下-NPY升高;他還可以促進(jìn)血小板聚集、白細(xì)胞粘附及 巨噬細(xì)胞活化而參 與免疫反應(yīng) 神經(jīng)肽Y廣泛分布于動(dòng)物中樞和外周神經(jīng)元及機(jī)體組織、器官和腺體內(nèi) ,影響動(dòng)物采食、激素分泌、心血管效應(yīng)、調(diào)節(jié)體溫、生物節(jié)律、性行為及動(dòng)物情緒等多種功能。 由于結(jié)構(gòu)中富含酪氨酸 ,故稱(chēng)為神經(jīng)肽酪氨酸 (NPY)。它的結(jié)構(gòu)與同是 36個(gè)氨基酸的胰多肽 (pp)和肽 YY(PYY)極其相似 ,故認(rèn)為同屬胰多肽家族。從分子結(jié)構(gòu)看 ,人、大鼠、豚鼠和兔序列安全一致 ,而牛、羊、豬 NPY序列與人的序列只相差一個(gè)氨基酸。 |
| 產(chǎn)品圖片 |
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(bs-0071R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded (Rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neuropeptide Y) Polyclonal Antibody, Unconjugated (bs-0071R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Formalin-fixed and paraffin-embedded rat brain tissue labeled with Rabbit Anti-NPY/Neuropeptide Y Polyclonal Antibody, Unconjugated(bs-0071R) at 1:400 followed byconjugation to the secondary antibody and DAB staining
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