| 產(chǎn)品編號(hào) | bs-0531R |
| 英文名稱 | Matriptase Rabbit pAb |
| 中文名稱 | 蛋白裂解酶（一種新的癌基因）抗體 |
| 別 名 | transmembrane protease, serine 6; 1300008A22Rik; IRIDA; MATRIPTASE2; TMPRSS6. |
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Specific References (1) | bs-0531R has been referenced in 1 publications.
[IF=2.608] Kim?S et al. Capsaicin induces atopic dermatitis-like manifestations through dysregulation of proteolytic system and alteration of filaggrin processing in rats. Exp Dermatol. 2018 Apr;27(4):332-339. WB ; Rat.
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| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 激酶和磷酸酶 合成與降解 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | ICC/IF=1:25
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 94 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Matriptase: 11-100/855 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
The protein encoded by this gene is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis. Subunit: Interacts with CDCP1. May interact with TMEFF1. Subcellular Location: Membrane; Single-pass type II membrane protein (Probable). Similarity: Belongs to the peptidase S1 family. Contains 2 CUB domains. Contains 4 LDL-receptor class A domains. Contains 1 peptidase S1 domain. SWISS: Q8IU80 Gene ID: 164656 Database links: Entrez Gene: 164656 Human Entrez Gene: 71753 Mouse Omim: 609862 Human SwissProt: Q8IU80 Human SwissProt: Q9DBI0 Mouse Unigene: 370885 Human Unigene: 34056 Mouse Unigene: 214123 Rat 蛋白裂解酶是第一種已在分子水平上辨明的、與癌癥相關(guān)的跨膜絲氨酸蛋白酶。他們的研究首次發(fā)現(xiàn)蛋白裂解酶可以誘發(fā)腫瘤。蛋白裂解酶是一種細(xì)胞膜蛋白，一直以來(lái)研究者們對(duì)這種蛋白裂解酶的致癌功能表示懷疑。 matriptase在多種上皮來(lái)源的腫瘤中均高水平表達(dá)，有學(xué)者認(rèn)為：matriptase作為一種癌基因，為跨膜蛋白而且位于細(xì)胞表面，部分位于胞漿。 |
| 產(chǎn)品圖片 |
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Matriptase) polyclonal Antibody, Unconjugated (bs-0531R) 1:25, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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