| 產(chǎn)品編號(hào) | bs-1161R |
| 英文名稱 | Rabbit Anti-GRM2 antibody |
| 中文名稱 | 促代謝型谷氨酸受體2抗體 |
| 別 名 | Metabotropic Glutamate Receptor 2; Glutamate Receptor Metabotropic 2; Gprc1b; AMPA selective glutamate receptor 2; GLUR 2; GLUR2; GLURB; Glutamate receptor metabotropic 2; Metabotropic Glutamate Receptor 2; GPRC1B; GRM 2; mGlu 2; MGlu2; MGLUR 2; Mglur2; GRM2_HUMAN. |
| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 G蛋白偶聯(lián)受體 G蛋白信號(hào) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Cow,Chicken,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 96kDa |
| 細(xì)胞定位 | 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GRM2: 801-872/872 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Function: Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization. Subunit: Interacts with GRASP. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Widely expressed in different regions of the adult brain as well as in fetal brain. Similarity: Belongs to the G-protein coupled receptor 3 family. SWISS: Q14416 Gene ID: 2912 Database links: Entrez Gene: 2912 Human Entrez Gene: 108068 Mouse Omim: 604099 Human SwissProt: Q14416 Human SwissProt: Q14BI2 Mouse Unigene: 121510 Human Unigene: 410822 Mouse Unigene: 424639 Mouse Unigene: 9681 Rat 谷氨酸受體-代謝能2；主要參與細(xì)胞信號(hào)轉(zhuǎn)導(dǎo)及突觸傳遞，在G蛋白偶聯(lián)受體蛋白信號(hào)轉(zhuǎn)導(dǎo)途徑中有一定的作用，并且有負(fù)調(diào)控腺苷酸環(huán)化酶的作用，為膜性蛋白，在胞膜、胞漿中表達(dá)。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse Cerebellum tissue lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Human SH-SY5Y cell lysates
Primary: Anti-GRM2 (bs-1161R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 96 kD
Observed band size: 120 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GRM2/GLUR2 Polyclonal Antibody, Unconjugated(bs-1161R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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