| 產(chǎn)品編號 | bs-1173R |
| 英文名稱 | TNFRSF18 Rabbit pAb |
| 中文名稱 | 糖皮質(zhì)激素誘導(dǎo)腫瘤壞死因子受體抗體 |
| 別 名 | Activation inducible TNFR family receptor; Activation-inducible TNFR family receptor; AITR; CD357; GITR; GITR D; GITR-D; GITRD; Glucocorticoid induced TNFR related protein; glucocorticoid-induced tnf receptor ligand; glucocorticoid-induced tnf receptors l |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 生長因子和激素 t-淋巴細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 24 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞膜 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse TNFRSF18: 201-214/228 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
TNFRSF18 or GITR (glucocorticoid-induced TNF receptor family-regulated gene) is a 25 kD TNF receptor superfamily member (also known as AITR and TNFRSF18) originally identified in the mouse by comparing untreated and dexamethasone-treated murine T cell hybridoma cells. Human GITR was subsequently identified by searching an expressed sequence tag database. GITR is expressed on activated lymphocytes and is upregulated by T cell receptor engagement. The cytoplasmic domain of GITR is homologous to CD40, 4-1BB and CD27 and has been shown to interact with TRAF 1-3, but not TRAF 5 or 6. GITR signaling has been shown to regulate T cell proliferation and TCR-mediated apoptosis, and to break immunological self-tolerance. GITR appears to be highly expressed on CD4+CD25+ T regulatory cells and has been shown to induce NF-kB activation through TRAF2/NIK signaling. GITR has been proposed to be involved in the development of regulatory T cells and to regulate the activity of Th1 subsets. GITR binds GITRL, a TNF superfamily ligand expressed on endothelial cells, dendritic cells, macrophages, and B cells. Function: Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway. Subunit: Binds to TRAF1, TRAF2, and TRAF3, but not TRAF5 and TRAF6. Binds through its C-terminus to SIVA1/SIVA. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Tissue Specificity: Expressed in lymph node, peripheral blood leukocytes and weakly in spleen. Similarity: Contains 3 TNFR-Cys repeats. SWISS: O35714 Gene ID: 21936 Database links: Entrez Gene: 8784 Human Entrez Gene: 21936 Mouse Omim: 603905 Human SwissProt: Q9Y5U5 Human SwissProt: O35714 Mouse Unigene: 212680 Human Unigene: 3180 Mouse Unigene: 482508 Mouse GITR屬于腫瘤壞死因子受體超家族（TNFRSF），GITR參與免疫調(diào)節(jié)信號，在T細(xì)胞的活化、凋亡,尤其是阻斷調(diào)節(jié)性T細(xì)胞（T regulatory cell）的抑制功能等活動中發(fā)揮主要作用。 |
| 產(chǎn)品圖片 |
Tissue/cell: human oral squamous cell carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩?? for 20 min;
Incubation: Anti-TNFRSF18 Polyclonal Antibody, Unconjugated(bs-1173R) 1:200, overnight at 4擄C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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