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Dopamine beta Hydroxylase Rabbit pAb (bs-0596R)  
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產(chǎn)品編號(hào) bs-0596R
英文名稱 Dopamine beta Hydroxylase Rabbit pAb
中文名稱 多巴胺β羥化酶抗體
別    名 DBH; DBM; Dopamine beta hydroxylase; Dopamine-beta-Hydroxylase; Dopamine beta monooxygenase; DOPO_RAT; DOPO_HUMAN.  
研究領(lǐng)域 腫瘤  神經(jīng)生物學(xué)  合成與降解  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Rat (predicted: Mouse)
產(chǎn)品應(yīng)用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 70 kDa
檢測(cè)分子量
細(xì)胞定位 細(xì)胞漿 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human DBH: 471-550/621 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Dopamine-beta-Hydroxylase (DBH), Dopamine can be transformed into Noradrenalin. Conversion of dopamine to noradrenaline. [SUBUNIT] Homotetramer composed of two non-covalently bound disulfide-linked dimers. [SUBCELLULAR LOCATION] Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (Potential). Belongs to the copper type II ascorbate-dependent monooxygenase family.

Function:
Conversion of dopamine to noradrenaline.

Subunit:
Homotetramer composed of two non-covalently bound disulfide-linked dimers (By similarity).

Subcellular Location:
Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen (By similarity).
Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (By similarity).

Tissue Specificity:
Chromaffin granules of the adrenal medulla and synaptic vesicles of the sympathetic nervous system.

Similarity:
Belongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 1 DOMON domain.

SWISS:
P09172

Gene ID:
1621

Database links:

Entrez Gene: 280758 Cow

Entrez Gene: 1621 Human

Entrez Gene: 13166 Mouse

Entrez Gene: 25699 Rat

Omim: 223360 Human

Omim: 609312 Human

SwissProt: P15101 Cow

SwissProt: P09172 Human

SwissProt: Q64237 Mouse

SwissProt: Q05754 Rat

Unigene: 223858 Human

Unigene: 591890 Human

Unigene: 167781 Mouse

Unigene: 87166 Rat



Dopamine-β-Hydroxylase(DBH)—多巴胺β羥化酶??梢詫⒍喟桶忿D(zhuǎn)化成去甲腎上腺素。是腎上腺素能神經(jīng)元的標(biāo)志。
產(chǎn)品圖片
Sample: Jurkat (Human) Cell Lysate at 40 ug Primary: Anti-Dopamine beta Hydroxylase (bs-0596R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOPO) Polyclonal Antibody, Unconjugated (bs-0596R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control(black line):SH-SY5Y. Primary Antibody (green line): Rabbit Anti-Dopamine beta Hydroxylase antibody (bs-0596R) Dilution:1ug/Test; Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line): Normal Rabbit IgG Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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