| 產(chǎn)品編號 | bs-1254R |
| 英文名稱 | Rabbit Anti-Apolipoprotein D antibody |
| 中文名稱 | 載脂蛋白D抗體 |
| 別 名 | Apolipoprotein D; APOD; APO D; APOD protein; APOD_HUMAN; Apolipoprotein D; Apo-D; ApoD. |
| 研究領(lǐng)域 | 腫瘤 心血管 細胞生物 免疫學(xué) 神經(jīng)生物學(xué) Alzheimer's |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Mouse,Rat (predicted: Human,Rabbit,Pig,Cow,Dog) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 19kDa |
| 細胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human APOD: 81-189/189 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Apolipoprotein D (APO D), a glycoprotein involved in the human plasma lipid transport system. It is a progesterone binding glycoprotein of 24,000 dalton monomer molecular size, is a constituent of high density lipoprotein in plasma. The function of apolipoprotein D in the metabolism of plasma lipoproteins is unclear but the observation that this protein forms complexes with lecithin: cholesterol acyltransferase has led to the suggestion that apolipoprotein D may be involved in cholesterol esterification and transport of substrates and products of the reaction. Apolipoprotein D is expressed in a range of normal tissues including axillary apocrine glands, adrenal cortex and corpus luteum. Peripheral nerves, pituitary, testis, cerebellum and renal tubes are also positive. APO D was first isolated in large quantity as GCDFP 24, the major protein component of most human breast cyst fluids. In recent studies, Apo D immunoreactivity has been shown to be an early indicator of prostate cancer and advanced primary prostate tumors. Function: APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts. Subunit: Homodimer. In plasma, also exists as a disulfide-linked heterodimer with APOA2. Subcellular Location: Secreted. Tissue Specificity: Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears. Post-translational modifications: N-glycosylatd. N-glycan heterogeneity at Asn-65: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); at Asn-98: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6 (minor). Similarity: Belongs to the calycin superfamily. Lipocalin family. SWISS: P05090 Gene ID: 347 Database links: Entrez Gene: 347 Human Omim: 107740 Human SwissProt: P05090 Human Unigene: 522555 Human 載脂蛋白D(apolipoprotein D,apoD)是一個糖蛋白,它最初是在人血漿的高密度脂蛋白中被分離出來的。apoD在結(jié)構(gòu)上與其他類型的載脂蛋白存在很大的差異,被歸入脂肪促成素家族。apoD可以與膽固醇、黃體酮、膽紅素等多種疏水性小分子結(jié)合。 apoD在多種脊索動物的各類組織中廣泛表達, 在脊椎動物中重要的生理功能。最近的研究表明,apoD可以作為多種癌癥及神經(jīng)系統(tǒng)疾病的早期診斷標記, apoD是一個多配體、多功能的蛋白質(zhì). |
| 產(chǎn)品圖片 |
Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti-Apolipoprotein D (bs-1254R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19 kD Observed band size: 30 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-APOD Polyclonal Antibody, Unconjugated(bs-1254R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
